Journal: Blood Coagulation & Fibrinolysis

Article Title: Characterization of antibody clones that bind exclusively to insoluble fibrin

doi: 10.1097/MBC.0000000000001171

Figure Lengend Snippet: Fibrin deposition stained with anti-fibrin antibodies in mouse pancreatic tissue samples. (a) Frozen tissue samples were incubated with 1 μg/ml of HRP-conjugated anti-fibrin antibodies. (b) FFPE tissue samples were incubated with 10 μg/ml of HRP-conjugated anti-fibrin antibodies. The arrows indicate islets of Langerhans. HE staining is shown in the leftmost column. Scale bar, 100 μm. HRP, horseradish peroxidase.

Article Snippet: A frozen human PDAC tissue section (OriGene Technologies, Maryland, USA) and a normal human pancreatic tissue section (BioChain, California, USA) were purchased commercially.

Techniques: Staining, Incubation

Journal: Blood Coagulation & Fibrinolysis

Article Title: Characterization of antibody clones that bind exclusively to insoluble fibrin

doi: 10.1097/MBC.0000000000001171

Figure Lengend Snippet: Fibrin deposition stained with anti-fibrin antibodies in human pancreatic tissue samples. (a) Frozen tissue samples were incubated with 1 μg/ml of HRP-conjugated anti-fibrin antibodies. (b) FFPE tissue samples were incubated with 10 μg/ml of HRP-conjugated anti-fibrin antibodies. The arrow indicates islets of Langerhans. HE staining is shown in the leftmost column. Scale bar, 100 μm. HRP, horseradish peroxidase.

Article Snippet: A frozen human PDAC tissue section (OriGene Technologies, Maryland, USA) and a normal human pancreatic tissue section (BioChain, California, USA) were purchased commercially.

Techniques: Staining, Incubation

Journal: Blood Coagulation & Fibrinolysis

Article Title: Characterization of antibody clones that bind exclusively to insoluble fibrin

doi: 10.1097/MBC.0000000000001171

Figure Lengend Snippet: Improvement of human pancreatic tissue staining with HRP-conjugated 99 and 1101 clones. Frozen tissue samples were incubated with 10 μg/ml of HRP-conjugated 99 or 1101 antibody. Scale bar, 100 μm. HRP, horseradish peroxidase.

Article Snippet: A frozen human PDAC tissue section (OriGene Technologies, Maryland, USA) and a normal human pancreatic tissue section (BioChain, California, USA) were purchased commercially.

Techniques: Staining, Clone Assay, Incubation

Journal: Endocrinology

Article Title: Olfactomedin 4 Deletion Improves Male Mouse Glucose Intolerance and Insulin Resistance Induced by a High-Fat Diet

doi: 10.1210/en.2018-00451

Figure Lengend Snippet: Olfactomedin 4 is expressed in human and mouse pancreatic islet β cells. (A) Immunofluorescence double staining of OLFM4 with insulin or glucagon in human pancreatic tissue sections. Scale bar, 50 µm. (B) Immunohistochemistry staining of Olfm4 and insulin in consecutive pancreatic tissue sections from WT and Olfm4−/− (knockout) mice. Scale bar, 50 µm. (C) Olfm4 mRNA expression in isolated islets from WT and Olfm4−/− mice by quantitative RT-PCR. Expression was normalized to β-actin expression. Data are expressed as mean ± SD (n = 3). (D) Western blot analysis of Olfm4 expression in isolated islets from WT and Olfm4−/− mice. β-Actin was used as a loading control.

Article Snippet: Human pancreatic tissue sections were purchased from US Biomax (Rockville, MD).

Techniques: Immunofluorescence, Double Staining, Immunohistochemistry, Staining, Knock-Out, Expressing, Isolation, Quantitative RT-PCR, Western Blot